The tie was the last drawn out of the velvet bag by 5 Live Breakfast presenters Nicky Campbell and Rachel Burden, who also drew three other enticing matches which will be played over the weekend of May 11 to 13.
Perpignan will host one quarter-final after Catalan Dragons were drawn at home to Warrington Wolves, who beat the French side in the semi-finals of the competition en route to winning at Wembley two years ago.
Last year's runners-up Leeds Rhinos are away to the only remaining Co-operative Championship club, Leigh Centurions in a rematch of the 1971 final.
Details of which ties will be televised live on BBC Sport and Sky Sports will be confirmed in the next 24 hours.
Carnegie Challenge Cup quarter-final draw:
Huddersfield Giants v London Broncos
Catalan Dragons v Warrington Wolves
Leigh Centurions v Leeds Rhinos
Wigan Warriors v St Helens
